Journal: EBioMedicine

Article Title: Immune-infiltration based signature as a novel prognostic biomarker in gastrointestinal stromal tumour

doi: 10.1016/j.ebiom.2020.102850

Figure Lengend Snippet: Kaplan-Meier analysis of RFS according to individual TIL populations in the TC. (a) CD3 + TC. (b) CD8 + TC. (c) CD45RO + TC. (d) Foxp3 + TC. (e) CD4 + TC. (f) NKp46 + TC. (g) CD20 + TC.

Article Snippet: Then, sections were incubated with primary antibodies against CD3 (Ventana, 790–4341; ready-to-use), CD8 (Dako, M7103; 1:100 dilution), CD45RO (Origene, TA807197; 1:100 dilution), CD4 (Abcam, ab213215; 1:300 dilution), Foxp3 (Abcam, ab22510; 1:200 dilution), NKp46 (Abcam, ab214468; 1:500 dilution), CD20 (Abcam, ab27093; 1:200 dilution), programmed death-1(PD-1) (Cell signaling Technology, 43,248; 1:50 dilution), and programmed death-ligand 1 (PD-L1) (Dako, M3653; 1:50 dilution) at 4 °C overnight.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Jianpi Yangzheng Xiaozheng decoction alleviates gastric cancer progression via suppressing exosomal PD-L1

doi: 10.3389/fphar.2023.1159829

Figure Lengend Snippet: JPYZXZ reduced the number of MDSCs in tumor bearing mice. (A,B) Percentage of MDSCs (CD45 + CD11b+Gr1+) in blood determined using flow cytometry. (C,D) Percentage of MDSCs (CD45 + CD11b+Gr1+) in tumors determined using flow cytometry. (E) Immunofluorescence staining for DAPI (nucleus), CD11b, Gr-1, and PD-L1 in tumor tissue sections from the xenograft tumor model. Data are represented as the mean ± SEM, n = 5 mice or n = 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The single-cell suspensions (1 × 10 7 cells/mL) were stained with fluorochrome-coupled antibodies AF488-CD45 (Elabscience, E-AB-F1136L), PE/Dazzle™594-CD11b (BioLegend, 101256), and APC-Gr-1 (BioLegend, 108412) for 30 min at 4°C.

Techniques: Flow Cytometry, Immunofluorescence, Staining

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: APC-Vio770 Recombinant human anti B220(clone REA755) , Miltenyi Biotec , Cat# 130–110-849; RRID:AB_2658286.

Techniques: Negative Control, Recombinant, Purification, Staining, Gene Expression, Lysis, Immunoprecipitation, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Blocking Assay, Cell Isolation, Software, Microscopy

Journal: Frontiers in immunology

Article Title: NLRP3 promotes allergic responses to birch pollen extract in a model of intranasal sensitization.

doi: 10.3389/fimmu.2024.1393819

Figure Lengend Snippet: FIGURE 4 Allergic sensitization to BPE is independent of inflammasome-induced caspase 1 activation. (A) Immunofluorescence staining of lung sections from C57BL/6 and Nlrp3-/- mice (Figure 3) against ASC (red) and CD45 (white). Dapi (blue) was used to stain cell nuclei. Scale bar is 20 µm. The dashed line marks the epithelial cell barrier. b= bronchiole. (B) Quantification of ASC+/CD45+ cells of 3 mice/group, 1 image/mouse as depicted in (A). (C) BPE-specific serum IgE was measured via antigen-induced cross-linking of Fcϵ-receptors on RBL-2H3 cells and subsequent degranulation and release of b-hexosaminidase (b-hex release). (D, E) Cytokines secreted into BALF (D) or by restimulated splenocytes (E) were measured by Multiplex technology. The data are presented as box plots of at least 5 mice per treatment group of 3 individual experiments. Each mouse is depicted as one data point. One-way ANOVA with Tukey’s post hoc test was performed to determine statistical significance. *p<0.05, **p<0.01.

Article Snippet: The following primary antibodies were used: ASC/TMS1 (D2W8U) Rabbit mAb (1:200, RRID: AB_2799736, Cell Signaling Technology), Goat Anti-Mouse CD45 Polyclonal antibody (1:100, RRID: AB_442146, R&D Systems).

Techniques: Activation Assay, Staining, Multiplex Assay